Yin Seal: Release

Yin Seal: Release is a powerful sealing technique developed by Tsunade. Over a period of time, Tsunade will focus and build up chakra in the seal mark on her forehead. The release command will disperse the seal and allow the stored chakra to pump back into her body. She uses the chakra to perform Creation Rebirth on herself or others through Katsuyu.

Trivia

 * After using the chakra from the Yin seal for her jutsu, if there is chakra left Tsunade may use it to re-form the seal as she did after her fight with Orochimaru.
 * Her Grandmother Mito Uzumaki has the same mark on her forehead, it is possible that she can use the Yin Seal as well.