Ajisai

was a young woman whose corpse replaced the First Animal Path in Nagato's Six Paths of Pain. In the anime, she is a kunoichi of Amegakure and a team-mate of Suiren.

Background
In the anime, some time after Nagato took control of Amegakure, Ajisai and her team-mates were sent to Konohagakure to join an early Chūnin Exam co-sponsored by Sunagakure. There, Ajisai and her teammates were randomly split up and sent to different rooms to take the written test. The proctor Shikamaru Nara explained that everyone only needs to answer one question on the test but their combined score with their team-mates will determine if they pass or fail the exam as they need exactly 100 points to pass.

Following her death, Ryūsui and Yūdachi delivered her corpse to the highest tower in Amegakure where Konan was waiting. Afterwards Nagato used her corpse to become his new Animal Path.

Appearance
The body of the second Animal Path belonged to a young girl, making it Pain's only female body. Like the first Animal Path, it was outfitted with chakra receivers: one above the nose, two on either side of the neck, a row of three vertically down each cheek, and two studs on each forearm. Its hair is dyed orange and tied together in a sort of onion-like bun at the top of the head, with spikes of hair protruding out of it.

Six-Tails Unleashed
In the anime, after Nagato tracked down the six-tails jinchūriki, she along with the other paths killed Tsurugi and the hunter-nin's he was with. Afterwards, she and the other paths confronted Utakata where he transformed into his Version 2 form. As the fight began, she summoned her chameleon to restrict the jinchūriki. After her summon is defeated, she collected the human path after he is killed and retreated. After the six paths of pain successfully capture Utakata, the Akatsuki extracted the Six-Tails from him.

Pain's Assault
She is seen as she approched Konoha, as Pain was getting ready to retrieve the Nine-Tails' jinchūriki, Naruto Uzumaki. On the outskirts of the village, the Animal Path is launched through the village's barrier by the Asura Path. As one of the Paths tasked with creating diversions, the Animal Path summons several animals to trample the village. Later mobilising with the Human Path, the Animal Path sets its sights upon the Konohagakure Intelligence Division where it tracks down a chakra receiver.

When Naruto returns to the village, the Animal Path summons the other Six Paths of Pain to the Deva Path's side and then calls forth several summons which are defeated by Gamabunta, Gamaken and Gamahiro. The Path is then subsequently decommissioned by Naruto using his Sage Technique: Spiralling Serial Spheres inside Bunta's mouth.

Trivia

 * is the Japanese term for the Hydrangea macrophylla.
 * She was the only female member of Nagato's Six Paths of Pain, and was also the only one to keep her voice instead of speaking through Yahiko's voice.