Yin Seal: Release

After focusing and storing an amount of chakra over a period of time in the Yin Seal on the user's forehead, the user releases the chakra with this technique, dispersing the seal and pumping the chakra back into the users body. Sakura Haruno states she is able to use the seal's power more liberally than Tsunade, due to the fact she doesn't have to hide her age. After storing and then releasing, the user's speed and strength increase dramatically, as Sakura demonstrated when she battled a small army of Ten-Tails Mini Clones.

Tsunade must use this stored chakra to perform the Creation Rebirth technique on herself and healed a vast amount of people through Katsuyu. Tsunade is also able to use the Strength of a Hundred Technique after releasing the seal.

Trivia

 * After using the chakra from the Yin Seal for her techniques, if there is any leftover chakra, Tsunade may use it to re-form the seal as she did after her fight with Orochimaru.
 * Tsunade's grandmother, Mito Uzumaki, had a similar diamond-shaped mark on her forehead. Although it is believed that Tsunade's seal was modelled after the mark, it is unknown whether her grandmother was able to use the same technique or not.