Tsunade

<Orochimaru. Her motives known, Tsunade revealed that she knew Orochimaru had lied when saying he'd leave Konoha alone, and that she couldn't bring herself to allow it, due to Nawaki and Dan having loved Konoha until the end of their days. While Later while deciding on Team Kakashi’s first mission, Tsunade received word from Sunagakure that the Akatsuki had abducted Gaara, now the Fifth Kazekage. Out of respect for the Konoha-Suna alliance, Tsunade sent Team Kakashi to assist. Aation in an effort to complete the mission as Sai was instructed to give Orochimaru ANBU data should they meet.

=== Twelve Guarmay also be after Sora. The elders and Danzō came to the conclusion they should confine or eliminate him. Before Tsunade could voice her refusal, Sora was found spying on them. Convinced that Tsunade wanted to eliminate him, Sora became

Hidan and Kakuzu Arc
Later, when two Akatsuki members entered the Land of Fire - namely Hidan and Kakuzu - Tsunade mobilised the Nijū Shōtai to look for and if possible, capture the Akatsuki duo. While capture never came to be possible, the Akatsuki members were both defeated. Tsunade later performed an autopsy on Kakuzu, and found Naruto's newest technique, Wind Release: Rasenshuriken to be too dangerous for use, based on the damage done to Kakuzu's body and Naruto's arm. She explained that the attack had struck at a cellular level, causing extensive damage to the both of them, and regretfully told Kakashi and Yamato not to allow Naruto to use the technique again.

Three-Tails Arc
In the anime, after finishing her autopsy on Kakuzu, Tsunade received a report from a deceased ANBU captain about Orochimaru and Sasuke's whereabouts. She called for Kakashi, and put him in charge of Team 8 to investigate. Because the mission involved a chance of finding Sasuke, Tsunade tried to keep Naruto from finding out about it. He found out anyway, so Tsunade didn't hesitate to send him as back-up.

Later, Tsunade received news that Team Kakashi had encountered the Three-Tails. Deciding that they needed to capture the beast quickly, as to not let it fall into enemy hands, Tsunade ordered Tenten, Rock Lee, and Ino Yamanaka to aid Team Kakashi in sealing the beast. Before sending them out, Tsunade made a large and special scroll to aid the team in sealing the beast. She also decided to summon and send out Katsuyu for further assistance. When the forces they sent continued to have problems with the sealing, Tsunade called them back to Konoha, and sent some ANBU to replace them. After she heard about the ANBU losing contact with Konoha, and the disappearance of the Three-Tails, Tsunade suspected that Akatsuki were somehow involved.

Itachi Pursuit Arc
After assigning Team Kakashi and Team Kurenai on a mission to find Itachi Uchiha in the hopes that it would lead them to Sasuke. Jiraiya later comes to inform her that he had found the location of the leader of Akatsuki. As both Shizune and Tsunade in shock demand that he tells them where he is so that they can immediately begin formulating a surprise attack. Jiraiya tells them both to not be so hasty and invites Tsunade for drinks, which she declines at first but ultimately ends up agreeing to it. While in Shushuya, Jiraiya reveals the details of the Akatsuki's leader and that he was in Amegakure and the difficulty of getting into the village. As Jiraiya declares that he would infiltrate the village on his own, Tsunade objects but Jiraiya reminds her that he is one of the Legendary Sannin and what that meant. After the two reminisce a bit, Tsunade tells Jiraiya to come back alive and if he needed help to send a toad and she'd come in person to back him up. On departing, Jiraiya tells her to remain cautious of Root.

Tsuchigumo Kinjutsu Arc
In the anime, word reaches Konoha that the Tsuchigumo clan's fort protecting a powerful forbidden technique was under attack. With Team Kakashi and Team Kurenai being the closest, Tsunade sent Katsuyu to inform them of their mission to aid the Tsuchigumo clan, in accordance with the peace treaty the Third Hokage made with them. Tsunade later receives word from the teams that they and the clan have considered destroying the technique to end the possible danger it can cause.

Invasion of Pain Arc
When word reaches Konoha of Jiraiya's death, Tsunade makes the village's top priority analyzing the clues Jiraiya left behind about Pain's identity. While the likes of Shizune, Ibiki, and Shikamaru are hard at work, Tsunade allows herself an opportunity to cry for her old friend. Naruto, just as badly affected by Jiraiya's death as Tsunade, went away to train, both of them knowing that he was Pain's next target.

When the invasion of Pain began, Tsunade tried to send word for Naruto to return home. She was stopped by the Konoha Council, who feared his capture by Akatsuki. However, Tsunade insisted that they must have faith in Naruto, along with the rest of the next generation, and so they relented. Tsunade began issuing orders for the village's defence, placing extra protection around those still working on Pain's secret. She summoned Katsuyu, and had her divide and seek out every villager so that she could protect and heal them remotely.

While sitting in the middle of a seal guarded by ANBU, transferring her chakra to Katsuyu, she was found by Pain's Deva Path, whom she recognised as one of the orphans Jiraiya trained in the past. Tsunade refused to tell it where Naruto was, proclaiming that Akatsuki was just a terrorist group. When Pain discovered Naruto's location through other means, the Deva Path resolved to make Tsunade see what Akatsuki's true goals were.

The Deva Path destroys the village. In the few moments between when the destruction began and ended, Tsunade poured all her chakra into Katsuyu in order to keep the inhabitants safe. In the aftermath, she confronted Pain, who attempted to kill her with his Asura Path. It was stopped by Naruto, who informed Tsunade that he would personally deal with Pain. Having used up almost all of her chakra, Tsunade's elderly form resurfaced, she then falls into a coma and does not see Naruto's victory over Pain.

Confining the Jinchūriki Arc
While Tsunade remained in a comatose state, Danzō was chosen to replace her as the Candidate Sixth Hokage. However, he was killed by the now-international criminal Sasuke Uchiha after the Kage Summit and the village prepared to make Kakashi Hatake the new Hokage, but Tsunade woke up before the appointment could be finalised. Tsunade learned of everything that happened while she was in her coma; the start of the Fourth Shinobi World War, the Shinobi Alliance, and the identity of Tobi. After replenishing her chakra levels, she convened a war council to prepare the village for the impending war. In the anime after the war council, she calls a private meeting in her temporary office as they discuss the impending war.

Three days later, Tsunade goes to Kumogakure to meet with the other heads of the Alliance. She is warmly greeted by the Fifth Mizukage while the Third Tsuchikage suggests she retire so that she can finish recuperating. When they get down to business, Tsunade learns that the other Kage have decided to send Naruto and Killer B into hiding to keep them safe from Akatsuki. Tsunade argues against this, believing that the jinchūriki will be valuable assets in the coming war. The other Kage insist that it's for the best and the Fifth Kazekage reminds her that she must also take others' views into consideration. She eventually agrees with their decision, albeit reluctantly. Later, she helps organise the joint armies, placing particular interest in the Logistical Support and Medical Division and the Intelligence Division. In the anime after the meeting with the other heads of the Alliance she request the Fourth Raikage that B teaches Naruto to achieve the control over the Nine-Tails.

Shinobi World War Arc
Later, Tsunade remains at headquarters with the Raikage and the Tsuchikage, providing input when needed. When the First Division engages several revived ninja, Tsunade is disturbed by the possibility that Dan may be among their numbers. She is later surprised by news of the Gold and Silver Brothers' Nine-Tails chakra.

When news reaches headquarters of Naruto and B's escape from their confinement, the Raikage asks Tsunade to help him deal with them. She summons Katsuyu and leaves it at headquarters, taking a mini-Katsuyu with her so that she and the Raikage can keep up to date on any urgent matters. When they intercept Naruto and B, Tsunade is amazed by Naruto's Nine-Tails Chakra Mode. Naruto appeals to Tsunade to let him and B pass, but she informs him that she can no longer show him special treatment and must do what is best for the Alliance. Naruto tries to escape but is stopped by the Raikage, who voices his willingness to kill Naruto and B if they would not submit. Tsunade however, tells him that she will not allow him to do so.

Turning to face the Raikage instead, she remembers Naruto's words, that he would not die until he became the Hokage, she argues that even if Naruto being killed delayed the enemy's plan, that it's unlikely that another jinchūriki will have the same success as Naruto has had with controlling the Nine-Tails, and going against A's instructions, allows him to pass through. When it seems that A will not be swayed, Tsunade is intent on fighting him, but as it turns out, A was testing Naruto and agrees to let him continue on to the battlefield when he proves himself.

Upon returning to headquarters, Tsunade reviews the data from the medical team and is disconcerted by what Madara Uchiha and Orochimaru had achieved with her grandfather's cells, and concludes that harvesting them is what had allowed Madara to live for so long. When A asks if there are any hints on how to defeat Madara, Tsunade replies by saying that Madara may in fact be the true definition of the word "immortal". She then tells Shikaku to inform Naruto and B about White Zetsu's transformation ability.

Having heard a report from Inoichi about the progress that the Alliance was making on the battlefield after Naruto's release, Shikaku comments that they were gaining the upper hand out there. At this, Tsunade smiles at A triumphantly. They are subsequently told, however, that a new chakra has been sensed in the Fourth Division's area:. This is confirmed to be Madara Uchiha which sends them into confusion. Mabui inquires about the reason behind the masked man assuming the name 'Madara', to which Tsunade responds that, even more than a name, Madara is an ideal which strikes fear into people from just the simple idea of him. A sudden tremor is felt, and after hearing the report from Inoichi that a meteorite had been dropped on the Fourth Division, and hearing Ao say that the lives of several shinobi had been lost, Tsunade decides to head out to the front lines of the battlefield.

Adamant about her decision, she tells Mabui to prepare her Heavenly Transfer Technique to transport her there. Mabui protests however, saying that her technique was not intended for use on living organisms. She comments that the Third Raikage was the only one able to withstand her technique because of his special body. Shikaku also tries to dissuade Tsunade, telling her that it was a huge gamble regardless, and that she wasn't so lucky when it came to gambling and that they should use the Flying Thunder God Technique instead.

Though she admits that she is bad at betting money, she argues that it was different when it came to her life, citing that as the reason why she’s still alive also claiming that they should use the Flying Thunder God Technique to transport Mei as she would simply use her Yin seal. Despite Mabui’s insistence, A told her to prepare the technique for two, as he would be accompanying Tsunade. Tsunade then says that they would need a way to deal with Madara's Fire Release techniques.

Mabui eventually caves, and uses her technique to send them to the battlefield. Upon their arrival, Tsunade attacks Madara immediately with a kick sending him flying while A dealt with Mū. Sustaining some injuries in transit, she releases the seal on her forehead and uses her Creation Rebirth technique to heal herself. Standing up alongside all the other Kage, she faces Madara prepared to fight.

As the battle with Madara begins, Tsunade states that first she will heal Ōnoki and Gaara's injuries, asking Mei and A to hold off Madara while she does so. As she's healing them, Naruto questions her on why she hasn't collapsed after using Creation Rebirth after she arrived. Tsunade tells him that this instance is different compared to the scale she had to use it on during Pain's invasion, as this time she was only healing her own injuries and they were nowhere near as bad as the injuries she healed during Pain's invasion. Naruto asks to be healed despite being a clone, so he can join the battle but Tsunade tells him there's no need for that - Ōnoki then goes on to explain that the war is no longer just about protecting him. After being completely healed, Ōnoki and Gaara join the battle just in time to protect everyone from Madara's attack. As Madara is downed by the Kage's combined assault, Naruto turns to Tsunade, but is told by Ōnoki that this battle is no longer Naruto's to fight. Tsunade tells Naruto that they can only win if they defeat both Madaras - she says that, while they protect him from the revived Madara on this side, he needs to protect them from Tobi. With that, Tsunade and the rest of the Kage give Naruto a message, telling him simply to "win" as the clone disperses.

Creation and conception
Tsunade's name is taken from the character of the same name from. The Tsunade in that story is vastly different in terms of personality, along with the fact that unlike her manga counterpart the original Tsunade was married to Jiraiya, however both were known to greatly excel in slug magic.

Trivia

 * The name means "mooring rope".
 * Tsunade shares the same blood type with her former team-mates, Jiraiya and Orochimaru.
 * Tsunade was the first female Hokage, as well as the first female Kage that appeared in the series.
 * Tsunade is first mentioned when Might Guy questions Rock Lee, Neji, and Tenten about their goals as genin. Tenten says that she wants to become a great kunoichi and follow the footsteps of the legendary Tsunade.
 * According to the Naruto databook:
 * Tsunade's hobby is gambling.
 * Tsunade wishes to fight Naruto.
 * Tsunade's favourite foods are chicken breast and sake, while her least favourite food is liver sashimi.
 * Tsunade has completed 1,256 official missions in total: 40 D-rank, 236 C-rank, 467 B-rank, 418 A-rank, 95 S-rank.
 * Tsunade's favourite phrase is, referring to getting rich without much effort.
 * While Tsunade's English anime voice actor is Debi Mae West, in most games she is voiced by Mary Elizabeth McGlynn, who is Kurenai Yūhi's voice actor. The few games where she is voiced by her anime voice actor are Naruto Shippūden: Legends: Akatsuki Rising, Naruto: Uzumaki Chronicles 2, Naruto: Clash of Ninja Revolution, and Naruto Shippūden: Clash of Ninja Revolution 3. In Naruto: Clash of Ninja Revolution 2, while being voiced by Mary Elizabeth McGlynn throughout the story-line, during game-play, most of Debi Mae West's voice-overs from the first game are still used.
 * Tsunade's grandmother, Mito Uzumaki had a similar diamond shape mark on her forehead. It is possible that Tsunade modelled her own seal after this.

Quotes

 * (When about to fight Naruto) "One of the Legendary Sannin taking on a snot-nosed genin… I oughta be ashamed of myself."
 * (To Jiraiya about Naruto) "Jiraiya, are you the one who taught him the Rasengan? Are you acting like a teacher by teaching him something he can't master? Don't give him any ideas! That's why this kid likes to daydream that he's going to become Hokage someday."
 * (To Orochimaru) "Why am I putting my life on the line? Because now… I am the Fifth Hokage of the Village Hidden in the Leaves!"
 * (To Orochimaru about Naruto) "I will die before I let you touch him!"
 * (To Shikamaru) "Grow up… Death comes with being a shinobi… There are times when death is hard to accept, but if you don't get over it, there's no future…"
 * (To the advisers) "People like Jiraiya… and Sarutobi, and Elder Chiyo of the Sand possessed something you two sorely lack. Do you know what that is!? …Faith! My grandfather, the First Hokage, believed in you and entrusted you with the village. Now it's your turn to believe in these children and entrust it to them!"
 * (To Pain) "I am the Fifth Hokage… You've trampled on our ancestors' treasure… their dream… And you will pay the price! As the Hokage, I will put a stop to you here and now!"
 * (To Shizune) "People become stronger because there are memories that they will not forget."
 * (About her bad gambling) "Only when it comes to money. My life is a different story. I'm still alive aren't I…?"