Board Thread:Versus Debates/@comment-30530312-20170403010718/@comment-31477317-20170407033930

Slug Princess00 wrote: Just because Tsunade and Konan are the only females in this battle, (besides Animal Path if you count her) that doesn't mean they automatically match-up as a suitable fight. Konan's Dance of the Shikigami is practically immune to physical attacks, and that isn't difficult to discover. I doubt Tsunade would waste any time on her when her skills could be used elsewhere and at a greater effect.

But animal path (chikusodo) was originally a male. So yes, Tsunade and Konan are the only females.