Yin Seal: Release

The Yin Seal: Release is a powerful sealing technique used by Tsunade. Over a period of time, Tsunade will focus and build up chakra in the seal mark on her forehead. The release command will disperse the seal and allow the stored chakra to pump back into her body. She has used this stored chakra to perform the Creation Rebirth technique on herself and healed a vast amount of people through Katsuyu.

Trivia

 * After using the chakra from the Yin seal for her jutsu, if there is any leftover chakra, Tsunade may use it to re-form the seal as she did after her fight with Orochimaru.
 * Tsunade's grandmother, Mito Uzumaki, had a similar diamond-shaped mark on her forehead. Although it is believed that Tsunade's seal was modelled after the mark, it is unknown whether her grandmother is able to use the same technique.