Ajisai

She was a young woman whose corpse replaced the First Animal Path in Nagato's Six Paths of Pain.

Appearance
The body of the second Animal Path belonged to a young girl, making it Pain's only female body. Like the first Animal Path, it was outfitted with chakra receivers: one above the nose, two on either side of the neck, a row of three vertically down each cheek, and two studs on each forearm. Its hair is dyed orange and tied together in a sort of onion-like bun at the top of the head, with spikes of hair protruding out of it. Her hair used to be long and brown.

Abilities
As the corpse through which the Animal Path ability was channelled, this body had the ability to summon the animals under the control of Pain such as a snake-tailed chameleon, a crab, a dog, and a bird. Along with these, it could also summon the other Six Paths of Pain corpses and Konan.

Tsuchigumo Kinjutsu Arc
In the anime, she was introduced here as the replacement for Nagato's First Animal Path. Together, she and the other paths captured Utakata and afterwards the Akatsuki extracted the Six-Tails from him.

Pain's Assault
The Second Animal Path is later seen as Pain was getting ready to retrieve the Nine-Tails' jinchūriki, Naruto Uzumaki. On the outskirts of the village, the Animal Path is launched through the village's barrier by the Asura Path. As one of the Paths tasked with creating diversions, the Animal Path summons several animals to trample the village. Later mobilising with the Human Path, the Animal Path sets its sights upon the Konohagakure Intelligence Division where it tracks down a chakra receiver.

When Naruto returns to the village, the Animal Path summons the other Six Paths of Pain to the Deva Path's side and then calls forth several summons which are defeated by Gamabunta, Gamaken and Gamahiro. The Path is then subsequently decommissioned by Naruto using his Sage Technique: Spiralling Serial Spheres inside Bunta's mouth.

Trivia

 * She was the only female member of Nagato's Six Paths of Pain, and was also the only one to keep her voice instead of speaking through Yahiko's voice.
 * Unlike the other paths, the anime did not elaborate on her life before becoming a Path of Pain.