Board Thread:Episode Discussions/@comment-25319479-20160901110335/@comment-27089028-20160905192304

Elveonora wrote: There is no evidence more TSB can be created after they are lost or destroyed, with the exception of Kaguya, who seemingly made one, but she used a slightly different technique and she is the progenitor of chakra after all.

All evidence points to them being limited to set amount and once that amount is reduced to zero, the user's ability to use TSB is lost. Technically the tree is the progenitor of Chakra and Chakra basically became a life force for humans so that's not really much of an excuse. If they are lost more can't be created I agree with as Naruto, Madara and Obito have a limit but Obito makes them appear in his initial Jinchuriki form and that looks like creating something not activating it. Madara's instantaneously burst into existence same as Naruto's but that's probably due to the fact they didn't go through an adjustment period like Obito did.