Board Thread:Episode Discussions/@comment-25319479-20160901110335/@comment-1512913-20160905190958

There is no evidence more TSB can be created after they are lost or destroyed, with the exception of Kaguya, who seemingly made one, but she used a slightly different technique and she is the progenitor of chakra after all.

All evidence points to them being limited to set amount and once that amount is reduced to zero, the user's ability to use TSB is lost.