The End

is episode 138 of the Naruto: Shippūden anime.

Synopsis
While Sasuke summons Kirin, and when he thinks that he finally defeats Itachi, Itachi starts to get up and shows his ultimate weapon, the Susano'o; Sasuke tries to draw out more chakra, using up the chakra that was suppressing Orochimaru, allowing the latter to emerge from Sasuke's Cursed Seal. However, Itachi permanently seals away Orochimaru using the Totsuka Sword. With no more jutsu at his disposal, Sasuke is left at Itachi's mercy as he goes right to him with the intent of taking the eyes of his little brother. But at the last second, Itachi taps Sasuke on the forehead and tells him "I'm sorry Sasuke, there won't be a next time" similar to the times he declined Sasuke's company in the past. He then dies, and Sasuke collapses shortly after.