The Risks of the Impure World Reincarnation

is episode 333 of the Naruto: Shippūden anime.

Summary
Madara questioned Tsunade if the Will of Fire is enough to take him down. Tsunade states the three rules of a medical-nin as she releases her seal. She then reveals a fourth rule — that only a medical-nin who has mastered the Strength of a Hundred Technique are allowed to break the three rules. The other Kage are stunned to hear this while Madara remains unimpressed with Tsunade's abilities. He activates his Susanoo ribcage as Tsunade attacked him, slightly cracking the Susanoo. Madara tries immolating Tsunade but is stopped by Mei, who also attacks. A and Ōnoki deals a heavy blow on Madara while Tsunade lands a kick on the latter. Madara commented on Tsunade's abilities. Madara noted that all that remains of Hashirama is his cells inside of him and all that's left of Izuna was his eyes that belong to him now. He further stated that the only thing that could be passed on is hatred. Meanwhile, at the other battlefield, Chōza reveals to Dan that Madara Uchiha has been reincarnated and that the five Kage are dealing with him. Dan was shocked to learn that Tsunade was now the Hokage. Dan expressed concern that they should instead, find the caster of the technique, noting that Hashirama is the only shinobi capable of defeating Madara. At the same time, Tsunade landed a devastating attack on Madara as his body begins to reform. Gaara attempted to bind and seal him although it was revealed to be a clone. The real Madara stabs Tsunade with his Susanoo Sword from below. Meanwhile in the forest, Itachi summons his crows to stop Sasuke from pursuing him. He later arrived at the cave where Kabuto was, seeing through his barrier and easily breaks through it with his Susanoo. Sasuke quickly caught up as he mistook Kabuto for Orochimaru. Kabuto attempted to manipulate Sasuke, only for him to note that he is his enemy. Tsunade revealed that she is able to heal without hand seals, leading Madara to note that the technique is similar to Hashirama's medical abilities. Ōnoki attacks Madara with Dust Release. However, Madara absorbed it through the Preta Path. Tsunade questions Madara's confidence, stating that the Kage forced him to use a wood clone. Madara admits five on one is a good match-up, thus creating twenty five wood cloones, with five each to battle each of the Kage. Sasuke attempts to kill Kabuto but is stopped by Itachi who noted that they need to not kill him in order to stop the technique. As Sasuke agrees to cooperate but for Itachi to answer his questions later, the two Uchiha brothers and Kabuto prepared themselves for a dynamic battle that is to come.