The New Akatsuki

is episode 348 of the Naruto: Shippūden anime.

Summary
Akatsuki's leadership — Yahiko, Nagato, and Konan — attend a meeting with the Amegakure leader Hanzō. During the meeting, members of Akatsuki discover that Konoha's Root division were attempting an assassination of the Akatsuki leaders attempted to stop them, but failed resulting in their deaths. Root and their leader Danzō Shimura are revealed to have plotted with Hanzō to end Akatsuki's rebellion, capturing Konan and giving Nagato an ultimatum; kill Yahiko or they will kill Konan. Choosing for him, Yahiko runs himself into Nagato's kunai killing himself. Enraged, Nagato uses the power of his Rinnegan to slaughter the Konoha and Ame shinobi.