In Attendance, the Six Paths of Pain

is episode 132 of the Naruto: Shippuden anime.

Synopsis
Jiraiya blinds one of the new Pains, and Fukasaku suspects that all three share the same eyes and vision, while Jiraiya realizes that each of the bodies has only one specific purpose. Jiraiya stalls for time while Fukasaku and Shima prepare a powerful genjutsu to defeat the bodies and are successful in doing so. As Jiraiya leaves, another Pain ambushes Jiraiya destroying his left arm. Jiraiya looks up to see six Pains, including the three killed before, leaving him horrified and wondering what Pain really is.