Mezu and his older twin brother, Gozu, were surgically altered to look like Kagen Uchiha, allowing them to take turns spying on the Uchiha clan by impersonating Kagen. When not in the village impersonating Kagen, Mezu wore a blue marked mask resembling a monkey. Both reported directly to Danzō about their findings for several years, but in the year leading up to the Uchiha Clan Downfall, they also helped Itachi Uchiha as he tried to avert the clan's attempted coup. On the night that Itachi wiped out the Uchiha clan, Mezu was impersonating Kagen. He offered no resistance to being killed by Itachi, well aware that Kagen's body must be among the dead; he felt it was his responsibility as the younger brother to die so that Gozu didn't need to.
Mezu and Gozu shared their Anbu mask design, a monkey. Despite the identical design, the colours were different; Mezu's mask was blue, while Gozu's mask was red.