"Night of Blood…!!" (血の夜…!!, Chi no Yoru…!!, Viz: Blood Night…!!) is chapter 539 of the original Naruto manga.
The Nine-Tails recalls Naruto's growth. Naruto says he'll do something about the Nine-Tails' hatred as well, one day. In turn, the Nine-Tails reproaches him for thinking he can do it. In the battlefield, Neji collapses from overuse of the Byakugan; so, Kiba has him go to the medical unit. Nagato and Itachi talk about Akatsuki, their dōjutsu and what their summoner has in mind for them. Itachi states that the combination of his Mangekyō Sharingan, coupled with Nagato's Rinnegan could be almost unstoppable. At the Logistical Support and Medical Division, medical-nin heal the wounded. Three jōnin-level medical-nin are later found dead. The sensors say they have not detected the enemy, and suspicion arises that someone is being controlled. Sakura argues that despite that, they must keep healing the shinobi. Neji opts to find the spy. Sakura receives a love letter from another shinobi but tells him she has feelings for someone else. Two medical-nin are later seen preparing to get back to work, but Neji then reveals himself as the killer. He attacks and presumably kills the two medical-nin.
- During Itachi's conversation with Nagato, his eyes are depicted without black sclerae.