At some point, Jiraiya went on a mission to retrieve the scroll, but failed to return it to Konoha. Before perishing, to make sure it didn't fall into the wrong hands, Jiraiya set a trap that only his student, Minato, could get through.
A group of Konoha-nin consisting of Minato, Kakashi, Guy, Kushina, Naruto, and Sakura went to retrieve the scroll. Naruto ended up triggering the trap, causing several toads to be summoned to protect the scroll. In the end, Minato was able to release the summoning and acquire the scroll, which was locked in a safe in Konohagakure. When Menma attacked Konoha, he burst through and tried to steal the scroll. It was destroyed by Menma when Naruto tried to use it when the moon turned red in colour.