"The Darkness of the Akatsuki" (暁の闇, Akatsuki no Yami) is episode 456 of the Naruto: Shippūden anime.
Jūzō asks Itachi about Duy, and tells him how he single-handedly defeated four of the Seven Ninja Swordsmen of the Mist. Jūzō and Itachi briefly discuss their specialties before the two carry out an assassination. Akatsuki gathers, and Pain fills the others in on Akatsuki's progress and growing reputation. He informs them about the plan to gather the Tailed Beasts. Jūzō is given a mission in the Land of Water, much to his displeasure. To prevent being taken by hunter-nin, Jūzō instructs Itachi to kill him if he is captured. The two complete their mission, but are pursued by Yagura and Kiri Anbu. Jūzō and Itachi fight them, taking out the Anbu, but Yagura channels Isobu's power. Yagura paralyses Itachi, but Jūzō frees him. Yagura aims a Tailed Beast Ball at them, which Jūzō blocks at the cost of his sword, part of which impales him. Itachi awakens Amaterasu, leaving Yagura at the brink of death. Jūzō dies from his injury. At the next Akatsuki gathering, Kakuzu's partner is also revealed to have been killed, by Kakuzu himself. Orochimaru tries to attack Itachi to take over his body, but is defeated. Pain assigns Kisame to be Itachi's new partner.
|Itachi Uchiha||Hideo Ishikawa||石川 英郎||Ishikawa Hideo|
|Fourth Mizukage: Yagura||Miyu Irino||入野 自由||Irino Miyu|
|Jūzō Biwa||Kosuke Goto||後藤 光祐||Gotō Kōsuke|
|Pain||Kenyu Horiuchi||堀内 賢雄||Horiuchi Ken'yū|
|Sasori (Hiruko)||Yutaka Aoyama||青山 穣||Aoyama Yutaka|
|Kakuzu||Takaya Hashi||土師 孝也||Hashi Takaya|
|Zetsu||Nobuo Tobita||飛田 展男||Tobita Nobuo|
|Tobi||Wataru Takagi||高木 渉||Takagi Wataru|
|Kabuto Yakushi||Nobutoshi Kanna||神奈 延年||Kanna Nobutoshi|
|Kisame||Tomoyuki Dan||檀 臣幸||Dan Tomoyuki|