After focusing and storing an amount of chakra over a period of time in the Strength of a Hundred Seal on the user's forehead, the user can release any amount of chakra with this technique by dispersing the seal and pumping the stored chakra back into the user's body. After the stored chakra from the Strength of a Hundred Seal is used, if there is any leftover chakra, the user may use it to re-form the seal.
After releasing the chakra from the seal, Tsunade and Sakura are able to use unique techniques. Firstly shown, they have used this stored chakra to perform the Creation Rebirth technique and heal a vast amount of people through Katsuyu. They both are also able to use the Ninja Art Creation Rebirth — Strength of a Hundred Technique after releasing the seal. Should the need arise, the user can even channel the released chakra into another person by extending their markings onto them through physical contact, as demonstrated by Sakura.
- The size and form of the release markings may depend on how much chakra is being released from the seal, as Sakura and Tsunade's seals had only spread around a small area.